R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The relative prominence of the secondary peaks varies significantly among R-PEs from different species. PE has three types of subunits: alpha (20,000 daltons), beta (20,000 daltons) and gamma (30,000 daltons). The molecular weight of intact PE has been found to be about 240,000 daltons. The alpha subunit of PE contains only the phycoerythrobilin (PEB) chromophore, while beta and gamma subunits contain both PEB and phycourobilin (PUB). Variability in the absorption spectra of PEs from various species reflects differences in the PEB/PUB ratio of the subunits. PE and closely related B-PE are the most intensely fluorescent phycobiliproteins, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution.
艾美捷藻红蛋白RPE背景:
从红藻中分离出R-藻红蛋白(PE)。其主要吸收峰在565nm处,次要峰在496和545nm处。次峰的相对突出度在来自不同物种的R-PE中显着不同。PE有三种类型的亚基:α(20000道尔顿),β(20000道尔顿)和γ(30000道尔顿)。已发现完整PE的分子量约为240000道尔顿。PE的α亚基仅含有藻红蛋白(PEB)发色团,而β和γ亚基含有PEB和藻红蛋白(PUB)。来自不同物种的PE的吸收光谱的变化反映了亚基的PEB/PUB比率的差异。PE和密切相关的B-PE是最强荧光的藻胆蛋白,其量子效率可能超过90%,并且在任何中等浓度的溶液中肉眼都容易看到其橙色荧光。
艾美捷藻红蛋白RPE光谱属性:
Extinction coefficient (cm -1 M -1) 1960000
Excitation (nm) 566
Emission (nm) 574
Quantum yield 0.82
艾美捷藻红蛋白RPE部分参考文献:
Tolypothrix种质对藻胆蛋白含量的评价
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期刊:Folia Microbiol(Praha)(2003):59
藻胆蛋白操纵子在色素适应蓝藻Calothrix PCC 7601中的协调表达:RcaD和RcaG的作用
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海洋光合原核生物原绿球菌的藻胆蛋白基因:遗传异质性快速进化的证据
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期刊:微生物学(2001):3171
藻胆蛋白-Fab缀合物作为单粒子荧光成像的探针
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期刊:细胞计数(2000):226
藻蓝蛋白裂解酶的新活性:藻蓝蛋白操纵子编码的蛋白PecE和PecF催化藻蓝蛋白的附着和向藻蓝蛋白的异构化
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期刊:FEBS Lett(2000):9