Electrochemical sensor for detecting pain reliever/fever reducer drug acetaminophen based onelectrospun CeBiOx nanofibers modified screen-printed electrode
Author and unit
Fei Caoa,b,∗, Qiuchen Dongc, Caolong Li a, Jun Chenc, Xiaoyu Mac, Yikun Huangc,Donghui Songc, Chenhui Ji d, Yu Lei b,c,∗
a Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing 211198, China
b Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
c Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA
d Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China
Sensors and Actuators B: Chemical
Joumal impact factor：5.401
Year：2017
Artcleinfo
Received 26 June 2017,
Revised 26 September 2017,
Accepted 28 September 2017,
Available online 29 September 2017.
Abstract
A series of functional CeBiOx nanofibers (NFs) with different Ce:Bi molar ratios were successfully preparedby a facile two-step synthetic procedure including electrospinning and calcination.After systematiccharacterization of their morphology and structure/composition using various advanced techniques, theas-synthesized CeBiOx NFs were employed to modify screen-printed electrodes (SPEs) for the determinationof fever reliever/pain reducer drug acetaminophen (AP) via both cyclic voltammetry (CV) anddifferential pulse voltammetry (DPV). The results showed that the electrocatalytic activity of CeBiOx NFswas heavily dependent on Ce:Bi molar ratio and the Ce0.75Bi0.25Ox NFs resulted in the highest electrooxidationsignal for AP among all Ce:Bi molar ratios tested in this study. Moreover, the Ce0.75Bi0.25Ox NFsmodified SPE showed a sensitivity of 360 A mM−1cm−2, a low detection limit of 0.2 M (signal to noiseratio of 3), a wide linear range up to 130 M for DPV-based AP detection, and good intra- and interelectrodereproducibility. Both the selectivity against uric acid, glucose, dopamine, ascorbic acid andthe accuracy of the developed sensor for real sample analysis were also investigated using commercialparacetamol tablets and AP-spiked human serum samples.
Fig. 1. (a) A representative SEM image of Ce0.75Bi0.25Ox NFs; (b) XRD patterns of Ce0.25Bi0.75Ox, Ce0.5Bi0.5Ox and Ce0.75Bi0.25Ox and CeO2 NFs, respectively
Fig. 2. (a) CV curves of the electrodes represented by unmodified screen-printed electrode (curve a), Ce0.25Bi0.75Ox NFs modified SPE (curve b), Ce0.5Bi0.5Ox NFs modified SPE(curve c), Ce0.75Bi0.25Ox NFs modified SPE (curve d) and CeO2 NFs modified SPE (curve e) in the presence of 50 M AP in 0.1 M PBS (pH 7.4) solution at a scan rate of 50 mVs−1;(b) CV curves of Ce0.75Bi0.25Ox NFs modified SPE in the presence of 100 M AP in 0.1 M PBS (pH 7.4) solution at different scan rates in the range of 20–200 mVs−1; (c) linearcalibration plots for the peak current versus square root of scan rate.
Fig. 3. (a) CV curves of the Ce0.75Bi0.25Ox NFs modified SPE in the absence and presence of different AP concentrations in the range of 50–500 M; Inset: a zoom-in view ofthe CV curve of Ce0.75Bi0.25Ox NFs modified SPE in the absence of AP. (b) the corresponding calibration plot for the peak current versus AP concentrations.
Fig. 4. (a) DPV curves of the Ce0.75Bi0.25Ox NFs modified SPE in 0.1 M pH 7.4 PBS solutions with successive additions of AP with the final concentrations from 2.5 M to130 M; (b) The corresponding calibration plot for the peak current versus AP concentrations. DPV parameters: increment potential of 5 mV, amplitude of 25 mV, pulse widthof 0.2 s, and pulse period of 0.5 s.
Fig. 5. (a) DPV curves of the Ce0.75Bi0.25Ox NFs modified SPE in 0.1 M pH 7.4 PBS solutions for the same concentrations (50 M) of UA, DA, AA, glucose and AP, respectively;(b) DPV curves of the Ce0.75Bi0.25Ox NFs modified SPE with successive additions of AP (10, 25, 40 and 55 M, respectively) in the presence of 10 M DA.T
Fig. 6. (a) The recovery experiment for the detection of commercial paracetamol tables; (b) The recovery experiment for the detection of AP-spiked human serum samples.
Conclusions
In summary, a series of novel CeBiOx NFs with different Ce:Bi molar ratios were synthesized by electrospinning followed by calcination. The results from CV and DPV support the claim that the Ce:Bi molar ratio has a significant effect on the electrocatalytic activity of CeBiOx NFs for AP. An AP electrochemical sensor based on Ce0.75Bi0.25Ox NFs modified SPE not only possesses high sensitivity towards AP detection, but also shows many other analytical merits, including low detection limit, a wide linear range, good selectivity, and excellent reproducibility. Meanwhile, the developed AP sensor was applied to detect AP in commercial paracetamol tablets and AP-spiked human serum samples with good accuracy. All these features indicate that Ce0.75Bi0.25Ox NFs modified SPE is a promising user-friendly electrochemical sensor for on-site determination of AP drug, a pain reliever and a fever reducer.
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