标题：
小鼠MI卵母细胞MPF活性及其相关基因的表达
接触镉。
镉暴露的小鼠MI卵母细胞MPF活性及其相关基因的表达。
句子翻译：
Research has revealed that cadmium can disrupt ovarian function; however, few reports have focused on MI oocytes meiotic progression, especially the activity of maturation promoting factor (MPF) and its related genes (Cdk1, Ccnb1, and Cdc25b) expression. In this study, GV oocytes cultured in vitro for 0, 6, and 9 hours with fifive groups (control and doses of 0.05, 0.5, 2.5, and 5 μM Cd). At the same dose of cadmium but difffferent exposure time: compared with 0h, Periodic changes in MPF activity were changed and continuously increased over time. The mRNA and protein expression of each MPF-related gene in difffferent cadmium dose groups were changed compared with that of 0h. At the same exposure time but difffferent dose of cadmium: compared with control group, MPF activity, mRNA and protein expressions of each MPF-related gene in all the cadmium exposure groups were increased at 9h after exposure. Cadmium maintains the high MPF activity in mouse MI oocytes during its meiotic process and disturbs the periodic change of MPF activity; meanwhile, cadmium exposure promotes the syntheses of MPF-related gene, which may be one of the molecular mechanisms for the maintenance of high MPF activity, and ultimately prevents the meiotic progression in oocytes.
摘要：研究表明，镉会破坏卵巢功能。但是，很少有报道关注MI卵母细胞的减数分裂进程，特别是成熟促进因子（MPF）及其相关基因（Cdk1，Ccnb1和Cdc25b）的表达。在这项研究中，GV卵母细胞与五十个组（对照组和0.05、0.5、2.5和5μMCd的剂量）在体外培养了0、6和9小时。在相同剂量的镉下，但暴露时间不同：与0h相比，MPF活性的周期性变化发生了变化，并随时间不断增加。与0h相比，不同镉剂量组各MPF相关基因的mRNA和蛋白质表达均发生了变化。在镉暴露相同但剂量不同的情况下：与对照组相比，所有镉暴露组在暴露后9h的MPF活性，各MPF相关基因的mRNA和蛋白质表达均增加。镉在减数分裂过程中在小鼠MI卵母细胞中维持较高的MPF活性，并扰乱了MPF活性的周期性变化。同时，镉的暴露促进了MPF相关基因的合成，这可能是维持高MPF活性的主要分子机制之一，并最终阻止了卵母细胞的减数分裂进程。
期刊Food and Chemical Toxicology 影响因子：4.679 发表于2017.12.26
词汇整理：（音标均为美音）
Cadmium /’kædmɪəm/ n. 镉
reveale /rɪ’vil/ v. 揭示
Ovarian /əʊˈveəri:ən/ adj. 卵巢的；子房的
meiotic [maɪ’ɒtɪk] adj. 减数分裂的
Cultured /’kʌltʃɚd/ v. 栽培；教化；培养
Doses [doʊs] n. 剂量；一剂；一服vt. 给 … 服药
Periodic [ˌpɪri’ɑːdɪk] adj. 周期的；定期的；回归的；间歇的，时而发生的
Continuously /kənˈtɪnjʊəslɪ/ adv. 连续不断地,接连地
protein /’prəʊtiːn/ n.蛋白
process /’prɑsɛs/ n. 过程；变化过程 v. 加工；处理；冲印；审核；列队行进
Disturb /dɪ’stɝb/ v. 扰乱
Syntheses /’sinθisi:z/ n. 综合体
Molecular /mə’lɛkjəlɚ/ adj. 分子的
mechanisms [‘mekənɪzəmz] n. 机械
Ultimately /’ʌltɪmətli/ adv. 最后，最终；基本上
progression /prə’ɡrɛʃən/ n. （事件的）连续；一系列；发展，进展；<数>级数
Maintenance [‘meɪntənəns] n. 维持，保持；保养，保管；维护；维修
maturation promoting factor (MPF) 成熟促进因子